Fig 1.
Detection of CheA2 under various culture conditions. The wild-type A3-68 Δbbe02 or B31A3 (15, 45) strain was cultivated under various conditions: UF conditions (23°C and pH 7.6), 34°C and pH 7.6, 37°C and pH 6.8, or FT conditions (switch from 23°C and pH 7.6 to 37°C and pH 6.8) (A) or in the presence of DMC (B) (1). Similar amounts of whole-cell lysates were analyzed by SDS-PAGE and then probed with CheA2, DnaK (a loading control), and OspC antibodies as previously described (57). Increased OspC expression was used as a marker for cells cultured in DMC.