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The A subunit has no detectable in vivo enterotoxicity, either alone or admixed with the B subunit. Groups of 3 BALB/c mice each were orally inoculated with 25 μg or 125 μg of LT or 125 μg of A, A plus B, or dmLT. G/C ratios were determined after 3 h. G/C ratios of >0.09 are positive. Neither A alone nor A admixed with B induced intestinal fluid secretion. ***, P < 0.001 compared to saline controls. The error bars indicate standard errors of the means.
