Skip to main content
. 2012 Jul;11(7):943–951. doi: 10.1128/EC.00120-12

Fig 1.

Fig 1

Low oxygen conditions affect the growth of C. neoformans. (A and B) Log-phase cells of B-3501A (A) or IFM 49144 (B) were grown under 20% O2 at 28°C and were then transferred to 1% O2 with 5% CO2. Cells were examined at the indicated time points. (Top) FACS analysis; (bottom) morphology of cells in the start culture at the log phase (0 h) and at 6 h of culture under 1% O2 (right). (C) IFM 49144 cells were grown under limited aeration conditions for 5 h (syn) and were released to 20% O2 for 60 min (0 h). The culture was transferred to 1% O2 with 5% CO2, and cells were examined at the indicated time points. (Top) FACS analysis; (bottom) cell morphology of cultures with limited aeration (left), 20% O2 for 60 min (center), and 1% O2 for 18 h (right). (D) FACS analysis of IFM 49144 cells. 0 h, cells were grown at 20% O2 for 60 min after release from arrest; 2 h, cells from 0 h were continuously grown for an additional 2 h under 20% O2; 18 h, synchronized cells were grown under 1% O2 for 18 h. Bars, 5 μm.