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Spot assay. (A) Threefold serial dilutions of B-3501A (starting with approximately 500 cells) were spotted onto YEPD alone or YEPD containing 0.5 μM latrunculin A. Cultures were incubated under the conditions indicated for 3 days, and photographs were taken. (B) Threefold serial dilutions of the T-DNA insertional mutants were spotted onto YEPD medium and were incubated under the conditions indicated for 3 days. The mutations are given on the left.
