Skip to main content
. 2012 Jul;11(7):943–951. doi: 10.1128/EC.00120-12

Fig 4.

Fig 4

Localization of GFP-Sac6. (A) Fluorescent images of GFP-Sac6 and Lifeact-RFP. Logarithmically growing cells expressing Lifeact-RFP (red) and GFP-Sac6 (green) were observed by fluorescence microscopy. (Lower left) Merged fluorescent image; (lower right) merged DIC microscopy image. An arrow indicates the emerging bud. (B) Brief treatment with LatA causes diminishing of the fluorescence signals of GFP-Sac6 and Lifeact-RFP. Log-phase cells were treated either with DMSO or with 100 μM LatA for 5 min and were observed under a microscope. (C) Localization of GFP-Sac6 and Cap1-RFP under 20% O2 (top) or 1% O2 (bottom) at 28°C for 18 h. The nuclei were stained blue by live staining with the dye Hoechst 33342. The arrow and arrowhead indicate the locations of the bud neck and vacuole, respectively. (D) Localization of Cap1-RFP in the sac6Δ strain under 20% O2 (top) or 1% O2 (bottom) at 28°C for 18 h. The arrowhead indicates the location of the vacuole. Bars, 5 μm.