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Protein topology prediction for CrΔ4FAD. The hydropathy plot (A) was generated using the TOPPRED program (http://mobyle.pasteur.fr/cgi-bin/portal.py?#forms::toppred) and is based on the algorithm of von Heijne (46). (B) Schematic overview of CrΔ4FAD topology. cTP, chloroplast transit peptide; Cytb5, cytochrome b5 domain; TMD, membrane-spanning domains, each composed of two transmembrane helices. The three histidine boxes and the heme binding motif are indicated.
