Table 2.
Oligonucleotide primers used in this study
| Primer name | Sequence (5′ to 3′)a | Purpose |
|---|---|---|
| NdeI-Ata-3.4-F | GGGCCCCATATGGGGCATAAAAAACGCAGTCCAAAAAACGGTG | Clone 5′ upstream region of ata |
| MluI-Ata-R | GGGCCCACGCGTAATCGAAGCATTCCAAATGACCTTGTAAAC | Clone 5′ upstream region of ata |
| MluI-Ata-F | GGGCCCACGCGTATTAATTAAGAAACTGGTTGGGAGGGCAAT | Clone 3′ downstream region of ata |
| XhoI-Ata-3.4-R | GGGCCCCTCGAGAGAGCTTCGGCTGATTGAACAAACTTTTAGG | Clone 3′ downstream region of ata |
| Ata-F-out | ATTTATTCAATTAGGATGCCGCCTCTTTTTTTGG | Confirm in-frame deletion of ata |
| Ata-R-out | CTAAGACTAAGCTTTGGATTGTTTTGTTCATCTC | Confirm in-frame deletion of ata |
| XbaI-Ata-C-F | GGGCCCTCTAGATTGGTCGTTGAGTTCG | Complementation of ata |
| SphI-Ata-C-R | GGGCCCGCATGCTTAATTAATCACACCACTAATACCA | Complementation of ata |
| Ata-SP-F | TATTTGTCTGAGAAGTTTTATGAATAAAGTTTACAAGG | Amplification of promoter and signal peptide of ata |
| Ata-SP-R | AGCAAAAGCATTTGGAGCAAAACAAATTACACCC | Amplification of promoter and signal peptide of ata |
| KpnI-TD-F | GGGCCCGGTACCAACAAAATTACCAATCTGGGTGATCAGTTACAA | Amplification of ata translocator domain |
| SphI-TD-R | GGGCCCGCATGCTTAATTAATCACACCACTAATACCAACGCGGAC | Amplification of ata translocator domain |
| SacI-SP-V5-6×His-F | GGGCCCGAGCTCTATTTGTCTGAGAAGTTTTATGAATAAAGTTTACAAG | Amplification of ata promoter-signal peptide-V5-6×His |
| KpnI-SP-V5-6×His-R | GGGCCCGGTACCATGGTGATGGTGATGATGACCGGTAC | Amplification of ata promoter-signal peptide-V5-6×His |
| NdeI-Ata-PD-F | GGGCCCCATATGGGGACAAATACCGAAGGGGGAATAG | Cloning passenger domain of ata |
| XhoI-Ata-PD-R | GGGCCCCTCGAGTTCTAAGGCCATGGCAGCG | Cloning passenger domain of ata |
| Ata-C2-F | ATTTATTCAATTAGGATGCCGCCTCTTTTTTTGG | Complementation of ata with its native promoter |
| Ata-C2-R | CTAAGACTAAGCTTTGGATTGTTTTGTTCATCTC | Complementation of ata with its native promoter |
| pBAD-R | CCGCCAGGCAAATTCTGTTTTATCAG | Confirm orientation of ata in pLVB-Ata vector |
a
Restriction sites are underlined.