Table 1.

Strains and growth rates in glucose-putrescine medium^a

Line no.	Strain	Descriptionb	Generation time (min ± SEM)
1	W3110	lacL8 lacIq	225 ± 7 (18)
2	CP2	ΔpatA	220 ± 11 (6)
3	CP6	ΔgabT	221 ± 14 (4)
4	CP14	ΔpuuE::cat	183 ± 10 (3)
5	CP36	ΔgabT ΔpuuE::cat	305 ± 8 (3)
6	BLS71	ΔpatA Δ(ydcSTUV-patD)::kan	240 ± 14 (9)
7	BLS73	Δ(ydcSTUV-patD)::kan	228 ± 7 (24)
8	BLS74	ΔpatA ΔpatD::cat	227 ± 8 (9)
9	BLS75	ΔpatD::cat	241 ± 13 (10)
10	BLS77	ΔpuuR::cat	NG, S
11	BLS79	ΔpuuP::cat	NG
12	BLS80	ΔpuuA::cat	NG
13	BLS81	ΔpuuA	348 ± 9 (6)
14	BLS82	ΔpatA ΔpatD	210 ± 9 (9)
15	BLS83	ΔpatD	241 ± 9 (12)
16	BLS84	ΔpuuR	121 ± 6 (12)
17	BLS85	ΔpuuP	NG
18	BLS88	ΔpatA ΔpuuA	NG
19	BLS89	ΔpuuC::cat	305 ± 15 (8)
20	BLS90	ΔpuuC	315 ± 12 (10)
21	BLS93	ΔpuuC ΔpatD	VSG
22	BLS96	ΔpuuB::cat	320 ± 10 (6)
23	BLS97	ΔpuuB	367 ± 18 (13)
24	BLS98	ΔpuuB patA::kan	NG
25	BLS99	ΔpuuC Δ(ydcSTUV-patD)::kan	VSG
26	BLS100	ΔpuuC ΔpatD::cat	VSG
27	BLS102	ΔgabD::cat ΔpatD	235 ± 10 (3)
28	BLS103	ΔpuuC ΔpatD ΔgabD::cat	NG
29	BLS104	ΔydcSTUV::cat	234 ± 7 (6)
30	BLS105	ΔydcSTUV	225 ± 9 (6)
31	BLS106	ΔpuuC ΔgabD::cat	443 ± 24 (3)
32	BLS108	ΔcsiD ΔpuuA	344 ± 16 (3)
33	BLS109	ΔpuuA ΔpatD	507 ± 33 (3)
34	BLS110	ΔpatA ΔpuuC	NG
35	PAJ2	ΔpuuD	NG
36	SR4	ΔgabD::cat	184 ± 23 (4)

^aNG, no growth; S, suppressors accumulate frequently on plates; VSG, very slow growth (10% increase per day) for several days, before a growth burst, which is assumed to be from accumulation of suppressors.

^bDeletions are assumed to be polar for genes with an antibiotic resistance gene indicated. In-frame deletions are assumed to be nonpolar, and there is no associated antibiotic resistance gene.