Fig 3.
M. smegmatis Δlgt mutant fails to attach the lipid anchor. (A) Triton X-114 phase partition of M. smegmatis strains expressing recombinant lipoprotein Mpt83. (B) Triton X-114 phase partition of M. smegmatis parental strain, Δlgt mutant, and Δlgt-lgtMtb complemented strain expressing recombinant LppX using anti-LprG antibody. Of note, anti-LprG antibody (provided by H. Bercovier) cross-reacts with LppX (see Fig. S1 in the supplemental material). (C) [14C]palmitic acid incorporation in the wild type and Δlgt mutant.