Fig 4.

Lipoproteins without a lipid anchor are not cleaved by LspA. Western blot analysis of whole-cell extracts of M. smegmatis strains expressing Mpt83 (A) or LppX (B). The signal peptide of lipoproteins Mpt83 and LppX accounts for approximately 2 kDa. The signal peptide is not cleaved in the lgt mutant in the case of LppX. The signal peptide of Mpt83 is cleaved in the Δlgt mutant, indicating Lgt-independent signal sequence cleavage. Western blot analyses were performed using anti-HA antibody and corresponding secondary antibody conjugated with horseradish peroxidase.