Fig 5.

Subcellular localization of Mpt83 and LppX in M. smegmatis Δlgt mutant and parental strains. Western blot analyses of fractionated M. smegmatis extracts are shown. Lipoproteins Mpt83 and LppX localize in the cell wall fraction in the parental strain but are absent from the cytoplasmic membrane and the cell wall fraction in the Δlgt mutant. Absence of lipoproteins in the cell envelope fractions suggests that lipoproteins in the Δlgt mutant are released into the supernatant (see Fig. 6 in the supplemental material).