Table 3.
Caldicellulosiruptor adhesins located downstream of type IV pilus gene clusters
| MCL groupa and/or gene locus | Protein property |
Protein abundancee in: |
||||||
|---|---|---|---|---|---|---|---|---|
| Length (aa) | Sizeb (kDa) | pIb | SigPc | TMDd | SB | SN | WC | |
| 1820 | ||||||||
| Athe_1871 | 642 | 70.1 | 5.37 | N | Y | 2.26E−03 | 1.07E−04 | 3.16E−06 |
| Calkr_0826 | 634 | 68.9 | 8.3 | N | Y | 5.37E−03 | 8.54E−04 | 7.07E−05 |
| Calkro_0844 | 642 | 69.6 | 5.18 | N | Y | 4.41E−03 | 8.77E−06 | 2.83E−06 |
| Calla_1507 | 634 | 69.0 | 8.02 | N | Y | 9.80E−03 | 2.32E−03 | 5.45E−04 |
| COB47_1678 | 642 | 69.8 | 5.13 | N | Y | NAf | NA | NA |
| Csac_1073 | 642 | 69.9 | 5.13 | N | Y | 4.29E−03 | 2.49E−03 | 5.99E−05 |
| 1653 | ||||||||
| Athe_1870 | 649 | 70.3 | 6.37 | N | Y | 2.04E−03 | 1.05E−05 | 3.13E−06 |
| Calhy_0908 | 638 | 71.0 | 5.8 | Y | Y | NDg | ND | ND |
| Calkr_0827 | 622 | 68.9 | 5.7 | Y | N | ND | ND | ND |
| Calkro_0845 | 649 | 70.5 | 7.02 | N | Y | 6.95E−04 | 8.67E−06 | 2.80E−06 |
| Calla_1506 | 628 | 69.5 | 6.01 | N | Y | ND | ND | ND |
| COB47_1675 | 649 | 70.3 | 5.72 | N | Y | NA | NA | NA |
| Csac_1074 | 649 | 70.4 | 5.58 | N | Y | 1.84E−04 | 1.75E−05 | 4.80E−05 |
| Calow_1589 | 667 | 71.7 | 9.23 | Y | N | 4.70E−03 | 1.60E−02 | 2.07E−04 |
| Calow_1590 | 900 | 100.2 | 5.12 | N | Y | 2.93E−04 | 7.64E−04 | 7.79E−05 |
OrthoMCL group numbers for orthologous Caldicellulosiruptor proteins (see Data Set S1 in the supplemental material). No orthologous groups were assigned to the two proteins detected from C. owensensis.
Predictions for molecular size and pI used the ExPASy Compute pI/Mw tool (http://web.expasy.org/compute_pi/) (19).
SigP, number of signal peptides; predicted using SignalP (http://www.cbs.dtu.dk/services/SignalP/) (67).
TMD, transmembrane domain; predicted using the TMHMM server (http://www.cbs.dtu.dk/services/TMHMM/) (36).
Protein abundance is reported as NSAF for each fraction screened. SB, substrate bound; SN, supernatant; WC, whole-cell lysate.
NA, protein abundance not available.
ND, not detected in protein fractions using proteomics.