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M. catarrhalis AniA-generated NO· reduces host cell viability. Viable HBE cell counts were determined in HBE cell culture (HBEC) or in cocultures with the wild-type M. catarrhalis O35E (wt) or O35E aniA mutant or norB mutant for 24 h in the following media: MEM* with (+) or without (−) 5 mM nitrite (A) or MEM* containing human hemoglobin (1 mg/ml) with (+) or without (−) 5 mM nitrite (B). ns, not significant.
