Fig 5.

M. catarrhalis AniA-generated NO· affects the levels of host proteins that play key roles in programmed death. HBE cells were cocultured with wild-type M. catarrhalis O35E (black bars) or aniA mutant cells (gray bars) in MEM* containing 1 mM nitrite for 24 h. Cell lysates were used to determine the relative levels of apoptotic proteins using a human apoptosis protein array kit. Values are means plus standard deviations from at least two repeated experiments (each in duplicate). pCasp3, precursor of caspase-3; PC, positive control.