Table 1.
Overall parameters and modeling summary for SAXS data
| Sample | Rg (nm)a | RgGNOM (nm)a | Rh (nm)b | Rg/Rhc | Dmax (nm)d | MWmone | MWexcf | χsg | χgh | χbi | χb_mcj | χfk |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AM-R1ab | 3.8 ± 0.1 | 4.2 ± 0.2 | 3.6 ± 0.5 | 1.2 | 15.5 ± 0.5 | 44,000 | 50,000 ± 5,000 | 1.0 | 1.0 | 1.2 | 1.1 | |
| R1ab-R2ab | 3.9 ± 0.1 | 4.2 ± 0.2 | 3.7 ± 0.5 | 1.1 | 14.5 ± 0.5 | 35,000 | 35,000 ± 5,000 | 1.0 | 1.0 | 1.1 | 0.9 | |
| AM-R1ab-R2ab | 4.2 ± 0.1 | 4.6 ± 0.2 | 4.3 ± 0.5 | 1.1 | 16.0 ± 0.5 | 61,000 | 59,000 ± 5,000 | 1.4 | 1.1 | 1.3 | 1.4 | 1.2 |
a
Rg, radius of gyration calculated using either Guinier approximation or the indirect Fourier transform package GNOM.
b
Rh, hydrodynamic radius measured by dynamic light scattering.
c
An Rg/R[infi]h ratio higher than 1 indicates flexibility in the system (35).
d
Dmax, maximum size, estimated using GNOM.
e
MWmon, expected molecular weight of monomeric proteins.
f
MWexc, concentration-independent estimation of MW from DAMMIN volume.
g
χs, discrepancy from average refined ab initio model generated by DAMMIN.
h
χg, discrepancy from reference GASBOR ab initio model.
i
χb, discrepancy from best BUNCH model.
j
χb_mc, discrepancy from multicurve BUNCH model.
k
χf, discrepancy from EOM ensemble model.