Table 1.
Sample | Rg (nm)a | RgGNOM (nm)a | Rh (nm)b | Rg/Rhc | Dmax (nm)d | MWmone | MWexcf | χsg | χgh | χbi | χb_mcj | χfk |
---|---|---|---|---|---|---|---|---|---|---|---|---|
AM-R1ab | 3.8 ± 0.1 | 4.2 ± 0.2 | 3.6 ± 0.5 | 1.2 | 15.5 ± 0.5 | 44,000 | 50,000 ± 5,000 | 1.0 | 1.0 | 1.2 | 1.1 | |
R1ab-R2ab | 3.9 ± 0.1 | 4.2 ± 0.2 | 3.7 ± 0.5 | 1.1 | 14.5 ± 0.5 | 35,000 | 35,000 ± 5,000 | 1.0 | 1.0 | 1.1 | 0.9 | |
AM-R1ab-R2ab | 4.2 ± 0.1 | 4.6 ± 0.2 | 4.3 ± 0.5 | 1.1 | 16.0 ± 0.5 | 61,000 | 59,000 ± 5,000 | 1.4 | 1.1 | 1.3 | 1.4 | 1.2 |
Rg, radius of gyration calculated using either Guinier approximation or the indirect Fourier transform package GNOM.
Rh, hydrodynamic radius measured by dynamic light scattering.
An Rg/R[infi]h ratio higher than 1 indicates flexibility in the system (35).
Dmax, maximum size, estimated using GNOM.
MWmon, expected molecular weight of monomeric proteins.
MWexc, concentration-independent estimation of MW from DAMMIN volume.
χs, discrepancy from average refined ab initio model generated by DAMMIN.
χg, discrepancy from reference GASBOR ab initio model.
χb, discrepancy from best BUNCH model.
χb_mc, discrepancy from multicurve BUNCH model.
χf, discrepancy from EOM ensemble model.