COM1 genome organization (left) compared to the P. furiosus reference sequence (NC_003413, right). Black tick marks on the outside of the circular diagrams represent full-length IS elements in both genomes, with two additional IS elements located at the boundaries on the COM1 genome between blocks A and D and blocks B and E. The block boundary between A and E indicates the start/end of the reference sequence and is just upstream of the origin of replication (ori) in both genomes. PF numbers associated with each block are ordered in the 5′-to-3′ direction of each arrow: A (red), PF0001 to PF0189; B (yellow), PF0190 to PF0347; C (green), PF0349 to PF0388; D (blue), PF0390 to PF1603′; E (purple), PF1603′ to PF2065. PF1603 is disrupted by an IS element in the COM1 genome.