Fig 3.

Maps of IS element insertions in both coding and potential regulatory regions of the COM1 genome compared to the NCBI reference sequence. The riboflavin synthase subunit alpha (PF0061) gene (A) and the region upstream of the cold-induced protein A (PF0190) (B) in COM1 are disrupted by IS elements. IS elements are represented by white arrows and are flanked by direct repeat sequences (boxed). A single copy of the direct repeat sequence is located at the same positions on the reference sequences, and the nucleotide sequence alignments are identical for the two genomic regions minus the IS element insertion and additional direct repeats. In the case of the riboflavin synthase gene, at the protein level the COM1 sequence has a premature stop codon (∼16 bp into the IS element).