Table 2.

Distribution of PepN activity

Location	Activitya of:
	ES114 (wild type)	pepN mutant	pepN+ mutant
Cytoplasm	6,760	22	8,121
Cell membrane	1,150	31	3,213
Periplasm	29	21	621
Outer membrane	36	32	54
Whole cells	10,150	995	14,323
Cell-free supernatant	328	291	970

^aResults are given as fluorescence units per OD₆₀₀ unit (5 × 10⁸ cells) above background. Values are representative of three separate experiments.