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Michaelis-Menten plots of P. aeruginosa Tpx activity versus peroxide substrates. The reaction mixtures contain 100 μM TrxA, 1.5 μM TrxB, 150 μM NADPH, 0.05 μM Tpx, and indicated concentrations of H2O2 (A), cumene hydroperoxide (CHP) (B), or t-butyl hydroperoxide (BHP) (C). The Km (app), kcat (app), and kcat (app)/Km (app) values were calculated using KaleidaGraph.
