Fig 1.

Primosomal proteins and Rep do not act directly on UV damage-arrested replication forks. (A) [¹⁴C]thymine-labeled cultures were pulse labeled with [³H]thymidine for 5 s, filtered, rinsed, and resuspended in nonradioactive medium and immediately UV irradiated with 27 J/m². (B) The fraction of total DNA, ¹⁴C (○), and nascent DNA, ³H (■), remaining in the culture at each indicated time point is plotted. Graphs represent averages from at least three independent experiments. Error bars represent one standard deviation. The initial values for ³H and ¹⁴C ranged from 1,300 to 4,000 cpm and 700 to 2,100 cpm, respectively, for all experiments.