Fig 3.
Primosomal proteins PriA and PriB, but not PriC, and Rep are required for the recovery of replication following UV irradiation. [3H]thymidine was added to [14C]thymine-prelabeled cultures for 2 min at the indicated times following either 27 J/m2 UV irradiation (filled symbols) or mock irradiation (open symbols) at time zero. The amount of total DNA at each time point relative to −10 min post-UV treatment, 14C (○), and DNA synthesis/2 min, 3H (□), is plotted. Graphs represent averages from at least three independent experiments. Error bars represent one standard deviation.