Table 1.

E. coli K-12 strains used

Strain	Relevant genotype	Reference or construction
SR108	λ− thyA36, deoC2 IN(rrnD-rrnE)1 rph	44
DY329	ΔlacU169 nadA::Tn10 gal490 λcI857 Δ(cro-bioA)	75
JC19008	priA2::kan dnaC809 (parent DM4000)	52
SS138	priB302	54
CL1070	ΔlacU169 nadA::Tn10 gal490 λcI857 Δ(cro-bioA) priC::cat	DY329 × PCR fragment (priC-cat primers)
CL1073	ΔlacU169 nadA::Tn10 gal490 λcI857 Δ(cro-bioA) rep::kan	DY329 × PCR fragment (rep-kan primers)
CL1631	ΔlacU169 nadA::Tn10 gal490 λcI857 Δ(cro-bioA) yjfC::kan	DY329 × PCR fragment (priB-postyjfC-kan primers)
CL1633	priB302 yjfC::kan	SS138 × P1 (CL1631)
Strains isogenic to SR108
HL921	recA::Tn10	14
CL530	pBR322	16
CL579	recF6206::tet	16
CL583	recF6206::tet pBR322	16
CL1102	priC::cat	SR108 × P1 (CL1070)
CL1105	rep::kan	SR108 × P1 (CL1073)
CL1122	priA2::kan	SR108 × P1 (JC19008)
CL1638	priB302 yjfC::kan	SR108 × P1 (CL1633)
CL1465	priC::cat pBR322	CL1102 transformed with pBR322
CL1467	rep::kan pBR322	CL1105 transformed with pBR322
CL2013	priB302 yjfC::kan pBR322	CL1638 transformed with pBR322