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Native XIP detection. (a) Extracted ion chromatograms (XICs) of the m/z range 876.40 to 876.44 of samples from the wild type UA159, the ΔcomS mutant SM065, and the synthetic ComS11-17. For relative abundance calculation, the highest peak in the m/z range 876.40 to 876.44 for each sample was set to 100%. (b) CID–MS/MS spectrum showing the protonated b and y ion fragments of the doubly protonated native peptide (m/z 438.72) in the wild-type sample.
