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Kinetic parameters of the thiol substrate of BagL
| Thiol | Mean parameter for thiol ± SDa |
||
|---|---|---|---|
| Km (μM) | kcat (min−1) | kcat/Km (μM−1 min−1) | |
| Cysteine | 15.4 ± 2.1 | 4,286 ± 142 | 277 ± 28 |
| Cysteinyl glycine | 8.4 ± 1.3 | 4,489 ± 163 | 536 ± 60 |
| GSH | 552 ± 78 | 6,129 ± 309 | 11.1 ± 3.9 |
The kinetic constants were calculated by nonlinear regression analysis. kcat values were calculated on the basis of a subunit Mr of 19,000. The values are expressed as means calculated from triplicate assays.
