Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype (phenotype) or characteristics | Source or reference |
|---|---|---|
| Strains | ||
| BH10C | E. coli cloning vector | 8 |
| UA159 | Transformable S. mutans isolate | 28 |
| MW04 | UA159 ΔoppD::spc (Spcr) | 17 |
| MW07 | UA159 ΔcomR::cat pWAR300 (Cmr Ermr) | 17 |
| MW17 | UA159 ΔcomS::spc pWAR304 (Spcr Ermr) | 17 |
| MW30 | UA159 pWAR312 (Ermr) | This study |
| Plasmids | ||
| pWAR304 | pFED761 derivative carrying PsigX-luxAB between the SalI and NotI sites; 6,466 bp (Ermr) | 17 |
| p7INT | Shuttle-suicide vector that integrates at the streptococcal bacteriophage T12 attB site (Ermr) | 18 |
| pWAR312 | Integrative derivative of p7INT carrying PsigX-luxAB between the XbaI and BamHI sites; 7,129 bp (Ermr) | This study |