Fig 8.

Localization of Sap and BslO in the envelope of B. anthracis secA2 and slaP mutants. B. anthracis strain Sterne and its mutants with mutational lesions in secA2 and slaP were fixed in 4% buffered formalin at 3 h postgermination. The localizations of Sap (A) and BslO (B) were observed with specific rabbit antisera as well as secondary antibody conjugates and counterstained with BODIPY-vancomycin to reveal the septal peptidoglycan. Images were obtained with a Leica SP5 Tandem Scanner Spectral 2-Photon confocal microscope (100× objective). Scale bars represent 2 μm.