Table 1.
Bacterial strains and plasmids used in this study
| B. anthracis strain or plasmid | Description | Reference |
|---|---|---|
| Strains | ||
| Sterne 34F2 | Wild-type (pXO1+ pXO2−) genome sequence | 49 |
| SN1 | Deletion of secY2 (BAS2547) (nucleotides 2549507-2550730) in 34F2 | This study |
| SN2 | secA2::Sp transposon insertion at nucleotide 890561 in 34F2 | |
| SN3 | secA2::Sp transposon insertion at nucleotide 890732 in 34F2 | This study |
| SN4 | secA2::Sp transposon insertion at nucleotide 891556 in 34F2 | This study |
| SN5 | secA2::Sp transposon insertion at nucleotide 891568 in 34F2 | This study |
| SN6 | secA2::Sp transposon insertion at nucleotide 891758 in 34F2 | This study |
| SN7 | secA2::Sp transposon insertion at nucleotide 892627 in 34F2 | This study |
| SN8 | secA2::Sp transposon insertion at nucleotide 892786 in 34F2 | This study |
| SN9 | secA2::Sp transposon insertion at nucleotide 893012 in 34F2 | This study |
| SN10 | secA2::Sp transposon insertion at nucleotide 893039 in 34F2 | This study |
| SN11 | Deletion of sap (BAS0841) (nucleotides 896758-899063) in 34F2 | This study |
| SN12 | Deletion of eag (BAS0842) (nucleotides 899843-902414) in 34F2 | This study |
| SN13 | Deletion of sap and eag (BAS0841 and BAS0842, respectively) (nucleotides 896758-902414) in 34F2 | This study |
| SN14 | Deletion of slaP (BAS0837) (nucleotides 889657-890538) in 34F2 | This study |
| SN15 | bslO::Sp transposon insertion at nucleotide 1703151 in 34F2 | 16 |
| Plasmids | ||
| pLM4 | Temp-sensitive pE194 replicon; Kanr | 38 |
| pJK4 | Pspac; lacI regulator; Kanr | 26 |
| pSN1 | pJK4 harboring secA2 | This study |
| pSN2 | pJK4 harboring secA2 and slaP | This study |
| pSN3 | pJK4 harboring slaP | This study |
| pSN4 | pJK4 harboring slaP with a C-terminal Strep tag | This study |
| pSN5 | pET15b harboring full-length secA2 with an N-terminal His tag | This study |
| pSN6 | pET15b harboring full-length slaP with an N-terminal His tag | This study |