Table 2.
Oligonucleotides used in this study
| Primer | Sequencea | Use(s) |
|---|---|---|
| smn11 F | TTTCCCGGGTGGTGATTGGACAGCTGAAA | sap |
| smn12 R | TTTGGTACCGCTAGCCAGGAACGTCTGGGAATGTT | sap |
| smn13 F | TTTGGTACCGCTAGCGAAGCAAAACCTGCAACAAA | sap |
| smn14 R | TTTGAGCTCTACAGCTGCAGAAGCACGAT | sap |
| smn17 F | TTTCCCGGGCTGCGACTACAGCAAAAGCA | eag |
| smn18 R | TTTGGTACCGCTAGCTTGCTGCTGTCATTGTACCTG | eag |
| smn19 F | TTTGGTACCGCTAGCTGAAAAATCAGTGGGGATTC | eag |
| smn20 R | TTTGAGCTCGACCAATCCATGCCTGCTAT | eag |
| smn23 F | TTTCCCGGGTTGGCACAAATAAATCAACCTTT | secY2 |
| smn24 R | TTTGGTACCGCTAGCTGTCGCCTCCTATATTTTTATCA | secY2 |
| smn25 F | TTTGGTACCGCTAGCTGTGGCACTTGAAACAATGAA | secY2 |
| smn26 R | TTTGAGCTCCAATTGTGAATTTGCACGTGACTGGT | secY2 |
| smn29 F | TTTTCTAGAATGCTGAATTCGGTAAAAAAGC | pSN1, pSN2 |
| smn30 R | TTTGGTACCTTATTGTACGTTTTCAGGAACACC | pSN1 |
| smn78 F | TTTTCTAGAATGTTATCATTCCTAAAAAAAGCTAAGAA | pSN3 |
| smn79 R | TTTTGGTACCTTAGTCTTGTGGTACAAGTGC | pSN2, pSN3 |
| smn86 F | TTTTCCCGGGGGAGCAAGAAGCTGATTTAATCG | slaP |
| smn87 R | CGAGCCTCTCTATGACTCGAGACTAGTAATCTTTCACCTCGTTATTGTACGTTTTC | slaP |
| smn90 F | ACGAGGTGAAAGATTACTAGTCTCGAGTCATAGAGAGGCTCGCCTC | slaP |
| smn91 R | TTTTGAATTCGTGCAAAGTGTAGGTGGTGAT | slaP |
| smn96 F | TTTTCCATGGCAGCAGCATGTTATCATTCCTAAAAAAAGCTAAGAAAAACG | pSN4 |
| smn97 R | TTTTGGTCCTCAGATTACTTCTCAAATTGAGGATGAGACCAGTCTTGTGGTACAAGTGCTTTC | pSN4 |
| smn137 | TTTTCTCGAGATGCTGAATTCGGTAAAAAAGC | pSN5 |
| smn138 | TTTTGGATCCTTATTGTACGTTTTCAGGAACACC | pSN5 |
| smn122 | TTTTCATATGTTATCATTCCTAAAAAAAGCTAAGAAAAACG | pSN6 |
| smn95 | TTTTGGATCCTCAGATTAGTCTTGTGGTACAAGTGCTTTC | pSN6 |
a
Primer sequences encompassing restriction enzyme cleavage sites are underlined.