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. 2012 Sep;78(17):6327–6336. doi: 10.1128/AEM.00892-12

Fig 5.

Fig 5

Mutation of psaN reduces persistence of colonization of P. stewartii in flea beetles. Beetles were allowed to feed on maize infected with two wild-type strains of P. stewartii (DC283 and DC440) or a psaN (DM5121) or sapD (DM5107) mutant strain. (B) Genetic complementation of the P. stewartii nonpolar psaN∷aphA3 mutant (DM7003) with a plasmid carrying wild-type PsaN (pDM3007) increases P. stewartii persistence in flea beetles. The number of viable P. stewartii cells was estimated as CFU from two or three beetle samples in each of three independent experiments. Data are mean log CFU per insect ± SE. Letters (a, b, and c) indicate significant differences in the number of CFU on day 10 (A) or 8 (B) (P < 0.05).