Fig 6.

The PSI-2 psaN is required for persistent transmission of P. stewartii by flea beetles. Beetles were allowed to feed on maize plants infected with wild-type (DC283), psaN-defective strains (DM5121 and DM7003), or a rescued psaN2∷aphA3/psaN⁺ strain [(DM7003(pDM3007)] for 2 days. Individual maize seedlings were then exposed to five beetles each from day 0 or day 8 after acquisition of bacteria for a 2-day transmission period. Data are means ± SE for five seedlings per treatment in three independent experiments. Asterisks indicate means significantly lower than unmarked bars (P < 0.05).