Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and/or phenotype | Source or reference |
|---|---|---|
| E. coli | ||
| DH10B | F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 endA1 recA1 deoRΔ (ara,leu)7697 araD139 galU galK nupG rpsL λ− | Gibco BRL |
| HB101 | F− thi-1 hsd20 (rB− mB−) supE44 recA13 ara-14 leuB6 proA2 lacY1 rpsL20 (Smr) xyl-5 mtl-1 | 5 |
| S17-1 | pro thi, chromosomal tra operon and recA from plasmid RP4 | 49 |
| P. stewartii | ||
| DC283 | A Nalr derivative of wild-type strain SS104 | 8 |
| DC440 | Wild type, isolated in 1999 in Wooster, OH | 10 |
| DM223 | wceG cpsA glycosyl transferase mutant; EPS partial mutant | 9 |
| DM5044 | DC283 yhiN∷mini-Tn5-gus Nalr Kmr | This study |
| DM5106 | DC283 sapD∷mini-Tn5-gus Nalr Kmr | This study |
| DM5107 | DC283 mtlA∷mini-Tn5-gus | This study |
| DM5108 | DC283 sicA∷mini-Tn5-gus Nalr Kmr | This study |
| DM5115 | DC283 ysaG∷mini-Tn5-gus Nalr Kmr | This study |
| DM5121 | DC283 psaN1∷mini-Tn5-gus Nalr Kmr | This study |
| DM5123 | DC283 lepA∷mini-Tn5-gus Nalr Kmr | This study |
| DM5125 | DC283 pilD∷mini-Tn5-gus Nalr Kmr | This study |
| DM 5126 | DC283 ospC∷mini-Tn5-gus Nalr Kmr | This study |
| DM5127 | DC283 kuP∷mini-Tn5-gus Nalr Kmr | This study |
| DM5130 | DC283 ackA∷mini-Tn5-gus Nalr Kmr | This study |
| DM7003 | DC283 psaN2∷aphA3 Nalr Kmr | This study |
| ESΔR | Hypermucoid, esaR | 3 |
| P. ananatis DC131 | Wild type, isolated from maize in Missouri in 1976 | 10 |
| D. dadantii EC16 | Wild type | 35 |
| Plasmids | ||
| pBluescript SK(+) | ColE1 α-lacZ Apr | Stratagene |
| pDM3007 | 1.4-kb BamHI/KpnI fragment containing Plac-ysaN+ cloned into pRK415; Tcr | This study |
| pLD55 | Suicide vector with R6K ori, α-lacZ, tetAR, Apr | 42 |
| pMM6 | pRK415 containing Plac-hrpL+ | 41 |
| pRK415 | IncP a-lacZ, Tcr | 34 |
| pRK2013∷Tn7 | ColE1 mob+ traRK2 ΔrepRK2 repE kan∷Tn7 Tpr Smr Spr | 19 |
| pUC18K | pUC18 with promoterless, terminatorless aphA cassette; Kmr | 39 |
| pUTmini-Tn5-gus | Mini-Tn5-gus in suicide vector pGP704; Apr Kmr | 22 |