Table 2.
Proportion of field-collected flea beetles (FLB) naturally infected with Pantoea stewartii
| Collection date | No. ofP. stewartii-positive FLB/total (%) | Gut region | No. positive/total (%) |
|---|---|---|---|
| June 2007 | 5/30 (16.7)a | NDc | ND |
| August 2007 | 15/60 (25.0)a | ND | ND |
| Sept. 2007 | 1/30 (3.3)a | ND | ND |
| Sept./Oct. 2007 | 3/91 (3.3)b | Foregut | 0/23 (0) |
| Midgut | 0/24 (0) | ||
| Hindgut | 2/23 (8.7) | ||
| Mtd | 1/21 (4.8) | ||
| April 2008 | 2/25 (8.0)a | ND | ND |
| Sept./Oct. 2008 | 1/48 (2.0)b | Foregut | 0/12 (0) |
| Midgut | 1/14 (0.07) | ||
| Hindgut | 0/11(0) | ||
| Mt | 0/11 (0) |
a
P. stewartii was detected using semiselective medium and verified using PCR.
b
P. stewartii was detected using immunofluorescence confocal microscopy of beetle guts.
c
ND, not determined.
d
Mt, Malpighian tubules.