Fig 3.

The response of Pseudomonas putida Fu1 to 2-FA is inducible. Aerated cultures were grown in 10 mM pyruvate (light gray bars) or 10 mM 2-FA (dark gray bars) at 30°C for 21 h. Cells were harvested, resuspended in CB, and placed in a U-tube chamber constructed by placing a U-shaped glass tube between a slide and a coverslip. Cells were allowed to chemotactically respond to either CB-, 2% CA-, or 2-FA-filled capillaries for 30 min at 30°C. Error bars represent the standard deviations for at least four independent assays conducted in triplicate. Means with the same letter are not significantly different (P < 0.05; one-way analysis of variance [ANOVA] interaction, Tukey multiple comparison test).