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. 2012 Sep;78(17):6035–6050. doi: 10.1128/AEM.01457-12

Fig 4.

Fig 4

Representative RT-PCR of Cronobacter iron acquisition systems under iron-replete (even-numbered lanes) and iron-depleted (odd-numbered lanes) conditions. (A) Cronobactin and shiF-viuB operons. Lane 1, 1 kb plus DNA ladder; lanes 2 and 3, 16S rRNA; lanes 4 and 5, viuB; lanes 6 and 7, shiF; lanes 8 and 9, iucA; lanes 10 and 11, iucB; lanes 12 and 13, iucC; lanes 14 and 15, iucD; lanes 16 and 17, iutA. (B) Enterobactin genes. Lane 1, 1 kb plus DNA ladder; lanes 2 and 3, fepA; lanes 4 and 5, entF; lanes 6 and 7, fepE; lanes 8 and 9, entC; lanes 10 and 11, fepB; lanes 12 and 13, entS; lanes 14 and 15, fepG; lanes 16 and 17, 16S rRNA. (C) TonB-dependent iron receptors. Lane 1, 1 kb plus DNA ladder; lanes 2 and 3, pfeA; lanes 4 and 5, fhuE; lanes 6 and 7, foxA; lanes 8 and 9, yncD; lanes 10 and 11, btuB; lanes 12 and 13, fcuA; lanes 14 and 15, fct; lanes 16 and 17, 16S rRNA.