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. 2012 Sep;78(17):6236–6250. doi: 10.1128/AEM.00897-12

Table 3.

Nodulation test on various leguminous plants and genotypic characterization by Southern hybridization of nodulation genes

Bradyrhizobium strains Nodulation ona:
 nod gene hybridizationb
A. americana G. max Ar. hypogaea V. radiata M. atropurpureum S. rostrata nodA nodB nodC
Divergent nod-containing strains
    SUTN1-2 + 0 + + + 0 YL CL JL
    SUTN1-3 + 0 + + + 0 Y CL JL
    SUTN1-4 + 0 + + + 0 YL CL JL
    SUTN1-6 + 0 + + + 0 YL CL JL
    SUTN1-8 + 0 + + + 0 YL CL J
    SUTN1-12 + 0 + + + 0 J CL JL
    SUTN1-13 + 0 + + + 0 YL CL JL
    SUTN2-1 + 0 + + + 0 YL CL J
    SUTN2-2 + 0 + + + 0 YL CL JL
    SUTN2-3 + 0 + + + 0 YL J JL
    SUTN2-4 + 0 + + + 0 YL CL JL
    SUTN4-3 + 0 + + + 0 YL CL J
    SUTN5-6 + 0 + + + 0 YL CL JL
    DOA1 + 0 + + + 0 YL CL JL
    DOA2 + 0 + + + 0 YL CL JL
    DOA3 + 0 + + + 0 YL CL JL
    DOA4 + 0 + + + 0 YL CL JL
    DOA6 + 0 + + + 0 N CL JL
    DOA7 + 0 + + + 0 YL CL JL
    DOA8 + 0 + + + 0 N CL JL
    DOA9 + 0 + + + 0 N CL JL
    DOA10 + 0 + + + 0 YL CL JL
nod-containing strains
    SUTN1-7 + 0 + + + 0 J J J
    SUTN1-9 + 0 + + + 0 J J J
    SUTN3-1 + 0 + + + 0 J J J
    SUTN4-1 + 0 + + + 0 J J J
    SUTN5-1 + 0 + + + 0 J J J
    SUTN5-3 + 0 + + + 0 J J J
    SUTN5-5 + 0 + + + 0 J J J
    SUTN6-1 + 0 + + + 0 J J J
    SUTN6-2 + 0 + + + 0 J J J
    SUTN7-1 + 0 + + + 0 J J J
    SUTN7-2 + 0 + + + 0 J J J
    SUTN8-1 + 0 + + + 0 J J J
    SUTN8-2 + 0 + + + 0 J J J
    SUTN9-1 + 0 + + + 0 J J J
    SUTN9-2 + 0 + + + 0 J J J
    SUTN9-3 + 0 + + + 0 J J J
    SUTN9-4 + 0 + + + 0 J J J
    SUTN9-5 + 0 + + + 0 J J J
    USDA110 0 + + + + 0 J J J
nod-independent strains
    BTAi1 0 0 + 0 0 0 N N N
    ORS278 0 0 + 0 0 0 N N N
a

+, positive with effective nodule; 0, negative.

b

Southern blot hybridization signals are presented in Fig. S3 in the supplemental material. J, signal obtained from B. japonicum USDA110 under a stringent condition; YL, signal obtained from strain SUTN6-2 (99% 16S rRNA similarity to B. yuanmingense) under a low-stringency condition; CL, signal obtained from strain SUTN7-2 (99% 16S rRNA similarity to B. canariense) under a low-stringency condition; JL, signal obtained from B. japonicum USDA110 under a low-stringency condition and from strains SUTN6-2 and SUTN7-2 under a stringent condition; N, not detected.