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High-performance liquid chromatography (HPLC) analysis of the hydrolysates of chloramphenicol (Cm) and florfenicol (Ff) by purified EstDL136. (A) Detection of Cm hydrolysis. Peaks 1 and 2 indicate p-nitrophenylserinol and Cm, respectively. (B) Hydrolysis of Ff was achieved following incubation with EstDL136. Peaks 3 and 4 represent the Ff hydrolysate and Ff standard, respectively. mAU, milli-absorbance unit.
