Table 1.
List of open reading frames from a metagenomic clone, pDL136, and closely related proteins in GenBank
| ORF | No. of amino acids | Closely related protein (accession no., organism) | % Identity |
|---|---|---|---|
| 1 | 187 | Hypothetical protein PP1Y_Mpl2761 (YP_004538189, Novosphingobium sp. PP1Y) | 54 |
| 2 | 580 | Amidohydrolase 3 (YP_001166016, Novosphingobium aromaticivorans DSM 12444) | 77 |
| 3 | 294 | Hypothetical protein Saro_3630 (YP_001166015, Novosphingobium aromaticivorans DSM 12444) | 68 |
| 4 | 214 | Short-chain dehydrogenase/reductase (YP_001264122, Sphingomonas wittichii RW1) | 44 |
| 5 | 442 | Major facilitator transporter (YP_001166021, Novosphingobium aromaticivorans DSM 12444) | 51 |
| 6 | 260 | Short-chain dehydrogenase/reductase (ZP_02892805, Burkholderia ambifaria IOP40-10) | 55 |
| 7 | 255 | Short-chain dehydrogenase/reductase (YP_001263771, Sphingomonas wittichii RW1) | 60 |
| 8a | 310 | α/β-Hydrolase domain-containing protein (YP_001166038, Novosphingobium aromaticivorans DSM 12444) | 58 |
| 9 | 129 | Hypothetical protein H16_B0647 (YP_728809, Ralstonia eutropha H16) | 30 |
| 10 | 246 | Short-chain dehydrogenase/reductase (YP_003277770, Comamonas testosteroni CNB-2) | 54 |
| 11 | 159 | AsnC family transcriptional regulator (YP_001166091, Novosphingobium aromaticivorans DSM 12444) | 68 |
| 12 | 163 | AsnC family transcriptional regulator (YP_001165999, Novosphingobium aromaticivorans DSM 12444) | 63 |
| 13 | 359 | Rieske (2Fe-2S) domain-containing protein (YP_001263791, Sphingomonas wittichii RW1) | 58 |
| 14 | 887 | TonB-dependent receptor (YP_495816, Novosphingobium aromaticivorans DSM 12444) | 53 |
a
orf8 encodes EstDL136, and the DNA sequence determined in pDL136 was partial.