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. 2012 Sep;78(17):6017–6026. doi: 10.1128/AEM.01099-12

Fig 2.

Fig 2

Production of PhaZBd in P. putida and qualitative mcl-PHA depolymerase activity determination. (A) Enzyme activity measured in mcl-PHA agar plates of 20 μg of protein of the soluble crude extract fraction of negative control P. putida KT2442(pIZ1016) (spot 1), P. putida KT2442(pIZBd1) (spot 2), P. putida TolA mutant AΩ negative control (pIZ1016) (spot 3), and P. putida TolA mutant AΩ(pIZBd1) (spot 4). (B) Enzyme activity measured in mcl-PHA agar plates of 20 μg of protein of the culture supernatants of P. putida KT2442(pIZBd1) (spot 1), positive control P. putida KT2442(pIZPZ) (spot 2), and P. putida TolA mutant AΩ(pIZBd1) (spot 3).