Table 3.
Proteins identified in MV released from P. putida DOT-T1E after different treatments
| Protein (source of homologous protein) | NCBI sequence no. | Molecular mass (kDa) | Probability scores after indicated treatmenta |
|---|---|---|---|
| TonB-dependent receptor (Pseudomonas stutzeri A1501) | gi148548751 | 88.8 | NaCl (206.3), 1-oct (55.7), HS (106.6), EDTA (183.1) |
| Ferripyoverdine receptor (Pseudomonas aeruginosa ) | gi170282622 | 85.1 | NaCl (243), 1-oct (179.8), HS (107.8), EDTA (72.6) |
| Elongation factor G (Pseudomonas mendocina ymp) | gi146308926 | 78.8 | NaCl (89.4), 1-oct (632.8), HS (391.1) |
| Outer membrane protein Opr86 (Pseudomonas aeruginosa PAb1) | gi296387845 | 72.9 | NaCl (158.9), 1-oct (91), HS (67.6), EDTA (134) |
| Putative lipoprotein (Pseudomonas fluorescens Pf-5) | gi152987388 | 69.6 | NaCl (60.9), 1-oct (66.3), HS (74.7), EDTA (111.5) |
| Chain A, quinoprotein ethanol dehydrogenase (Pseudomonas aeruginosa ) | gi10120672 | 64,0 | NaCl (152.8), 1-oct (768.1), HS (461.4), EDTA (375.2) |
| CopA family copper resistance protein (Pseudomonas aeruginosa 152504) | gi313107602 | 61.8 | HS (62.3), EDTA (214.3) |
| Chaperonin GroEL (Pseudomonas mendocina NK-01) | gi152986611 | 57.0 | NaCl (165.9), 1-oct (1168), HS (127.9), EDTA (265.4) |
| OprD family porin (Pseudomonas entomophila L48) | gi104781455 | 49.4 | NaCl (109.8), 1-oct (136.3), HS (131.3), EDTA (99.7) |
| Putative outer membrane protein precursor (Pseudomonas aeruginosa UCBPP-PA14) | gi116049240 | 45.5 | NaCl (136.7), HS (77.4), EDTA (97.7) |
| OprE3 (Pseudomonas aeruginosa ) | gi107102299 | 44.8 | NaCl (171.3), 1-oct (72.7), EDTA (135.5) |
| Elongation factor Tu (Pseudomonas putida KT2440) | gi26987181 | 43.5 | 1-oct (239.1), HS (161.4), EDTA (136.7) |
| Bmp family protein (Pseudomonas syringae pv. actinidiae strain M302091) | gi32455881 | 38.7 | HS (103.6), EDTA (79.3) |
| Extracellular solute-binding protein (Pseudomonas mendocina ymp) | gi146308575 | 36.7 | NaCl (141.6), EDTA (136.7) |
| OprF (Pseudomonas sp. MFY72) | gi37704628 | 34.5 | NaCl (113.1), 1-oct (122.7), HS (161.3), EDTA (133.5) |
| Periplasmic binding protein, putative (Pseudomonas putida KT2440) | gi26990659 | 33.2 | 1-oct (94), EDTA (75.6) |
| Small protease (Pseudomonas aeruginosa ) | gi54072636 | 20.8 | HS (105.9), EDTA (95) |
| Outer membrane protein OmpH (Pseudomonas fluorescens WH6) | gi152985326 | 19.1 | HS (91.6), EDTA (107.4) |
| Peptidoglycan-associated lipoprotein (Pseudomonas fluorescens Pf-5) | gi70732082 | 17.7 | NaCl (168.5), 1-oct (117.1), HS (123.8), EDTA (150.6) |
| Lipopolysaccharide transport periplasmic protein LptA (Pseudomonas fulva 12-X) | gi107099955 | 17.0 | NaCl (59.7), 1-oct (62.2), HS (149.3) |
| Riboflavin synthase subunit beta (Pseudomonas mendocina ymp) | gi146308868 | 16.5 | NaCl (76.5), 1-oct (84.2), HS (89.6), EDTA (79.2) |
| OprL (Pseudomonas fluorescens) | gi259090537 | 16,5 | NaCl (78.7), 1-oct (131.7) |
| 50S ribosomal protein L22 (Pseudomonas aeruginosa PAO1) | gi15599454 | 11.9 | NaCl (93.7), 1-oct (205), HS (121), EDTA (240.1) |
a
NaCl, 2 M; HS, heat shock (55°C); 1-oct, 1.25 mM 1-octanol; EDTA, 10 mM.