Fig 4.

MhbT transports [carboxyl-¹⁴C]3-hydroxybenzoate in the heterologous expression system. P. putida PaW340 and its transconjugants were grown in LB with 3-hydroxybenzoate and IPTG induction at 30°C. The same experiments were repeated three times, with the same trends. 3HBA, 3-hydroxybenzoate; ■, P. putida PaW340; ●, P. putida PaW340[pZWXY002] (carrying the mhb gene cluster); ▲, P. putida PaW340[pZWXY003] (carrying the mhb gene cluster with truncated mhbT); ▼, P. putida PaW340[pZWXY001,pZWXY003] (carrying the mhb gene cluster with truncated mhbT and recombinant MhbT); ○, P. putida PaW340[pZWXY003,pZWXY007] (carrying the mhb gene cluster with truncated mhbT and the recombinant MhbT D82A mutant); ♢, P. putida PaW340[pZWXY003,pZWXY008] (carrying the mhb gene cluster with truncated mhbT and the recombinant MhbT V311W mutant); □, P. putida PaW340[pZWXY003,pZWXY009] (carrying the mhb gene cluster with truncated mhbT and the recombinant MhbT D314A mutant).