Fig 7.

Altered pigA transcription in glucose-rich medium in the WT but not a gdhS mutant. (A and B) β-Galactosidase activity was measured from a chromosomal pigA promoter-lacZ fusion that measured expression of the prodigiosin biosynthetic operon after growth for 20 h. (A) β-Galactosidase activity was measured from WT (CMS376) and gdhS mutant (CMS1083) cultures grown in LBG (+ glucose) and LB (− glucose). Data from eight biological replicates per condition from two separate experiments are shown. (B) Expression of the PpigA-lacZ fusion was inhibited by growth in d-glucono-1,5-lactone at >20 mM in both the WT and the gdhS (rig-1 with restored crp) mutant strains. A representative experiment using 4 biological replicates per condition is shown and was consistent with a repetition of the experiment on a different day. Error bars indicate one standard deviation, and the asterisk indicates that the WT + glucose condition produced a different amount of β-galactosidase activity than all other conditions, as determined by an ANOVA with a Tukey posttest (P < 0.05).