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. 2012 Sep;78(17):6225–6235. doi: 10.1128/AEM.01778-12

Table 1.

Strains and plasmids used in this study

Strain or plasmid Description Reference or source
Strains
    Saccharomyces cerevisiae
        InvSc1 MATa/MATα leu2/leu2 trp1-289/trp1-289 ura3-52/ura3-52 his3-Δ1/his3-Δ1 Invitrogen
    E. coli
        SM10 λpir thi thr leu tonA lacY supE recA::RP4-2Tc::Mu pir 34
        S17-1 λpir thi pro hsdR hsdM+ ΔrecA RP4-2::TcMu-Km::Tn7 pir 34
    S. marcescens
        CMS376 WT Presque Isle Cultures strain no. 3611 46, 47
        Nima Pigmented laboratory strain Pryce Haddix
        CHASM Pigmented environmental isolate 27
        CMS613 crp-1 (insertion mutation), null allele 28
        CMS1083 gdhS (rig-1) with restored crp gene This study
        CMS1687 Δcrp has crp-Δ4 deletion mutation, null allele 27
        CMS1758 pigA::lacZ (pStvZ3 insertion at pigA) 27
        CMS2526 gdhS (rig-1) pigA::lacZ (pStvZ3 insertion at pigA) This study
Plasmids
    pMQ118 nptII rpsL oriT oriR6K URA3 CEN6/ARSH4 47
    pMQ125 aacC1 oriT orip15a URA3 CEN6/ARSH4 PBAD-lacZα 47
    pMQ132 oripBBR1 aacC1 oriT URA3 CEN6/ARSH4 47
    pMQ185 pMQ125 + crp This study
    pStvZ3 oriR6K lacZ nptII promoter probe; oriT URA3 CEN6/ARSH4 27
    pMQ268 pStvZ3 + pigA internal fragment 27
    pMQ335/pgdhS pMQ132 + gdhS This study
    pMQ339/pgdhS pMQ335 with aphA-3 replaced by aacC1 from pMQ132 This study
    pMQ346/pgcd pMQ132 + gcd This study
    pMQ372/pubiB pMQ132 + ubiB This study