Strains |
|
|
Saccharomyces cerevisiae
|
|
|
InvSc1 |
MATa/MATα leu2/leu2 trp1-289/trp1-289 ura3-52/ura3-52 his3-Δ1/his3-Δ1
|
Invitrogen |
E. coli
|
|
|
SM10 λpir |
thi thr leu tonA lacY supE recA::RP4-2Tc::Mu pir
|
34 |
S17-1 λpir |
thi pro hsdR hsdM+ ΔrecA RP4-2::TcMu-Km::Tn7 pir
|
34 |
S. marcescens
|
|
|
CMS376 |
WT Presque Isle Cultures strain no. 3611 |
46, 47
|
Nima |
Pigmented laboratory strain |
Pryce Haddix |
CHASM |
Pigmented environmental isolate |
27 |
CMS613 |
crp-1 (insertion mutation), null allele |
28 |
CMS1083 |
gdhS (rig-1) with restored crp gene |
This study |
CMS1687 |
Δcrp has crp-Δ4 deletion mutation, null allele |
27 |
CMS1758 |
pigA::lacZ (pStvZ3 insertion at pigA) |
27 |
CMS2526 |
gdhS (rig-1) pigA::lacZ (pStvZ3 insertion at pigA) |
This study |
Plasmids |
|
|
pMQ118 |
nptII rpsL oriT oriR6K URA3 CEN6/ARSH4
|
47 |
pMQ125 |
aacC1 oriT orip15a URA3 CEN6/ARSH4 PBAD-lacZα
|
47 |
pMQ132 |
oripBBR1 aacC1 oriT URA3 CEN6/ARSH4
|
47 |
pMQ185 |
pMQ125 + crp
|
This study |
pStvZ3 |
oriR6K lacZ nptII promoter probe; oriT URA3 CEN6/ARSH4
|
27 |
pMQ268 |
pStvZ3 + pigA internal fragment |
27 |
pMQ335/pgdhS
|
pMQ132 + gdhS
|
This study |
pMQ339/pgdhS
|
pMQ335 with aphA-3 replaced by aacC1 from pMQ132 |
This study |
pMQ346/pgcd
|
pMQ132 + gcd
|
This study |
pMQ372/pubiB
|
pMQ132 + ubiB
|
This study |