Table 3.
Strain phenotypes in glucose-rich medium
| Straina | Culture colorb | Culture pHc | Mutation (insertion site)d |
|---|---|---|---|
| Δcrp | White | 3.54 ± 0.08 | NA |
| rig-1 | Dark red | 5.99 ± 0.09 | gdhS (972) |
| rig-2 | Dark red | 5.29 ± 0.14 | gdhS (−2) |
| rig-5 | Dark red | 6.23 ± 0.03 | gdhS (2181) |
| rig-10 | Dark red | 6.05 ± 0.03 | gdhS (123) |
| rig-11 | Dark red | 6.05 ± 0.02 | gdhS (2008) |
| rig-12 | Bright pink | 3.51 ± 0.05 | hexS (213) |
| rig-13 | Dark red | 6.16 ± 0.03 | pqqE (172) |
| rig-A2 | Dark red | 6.27 ± 0.17 | gdhS (121) |
| rig-A6 | Dark red | 6.31 ± 0.10 | gdhS (2340) |
| rig-A14 | Dark red | 6.30 ± 0.23 | gdhS (121) |
| rig-A21 | Bright pink | 3.92 ± 0.37 | ubiB (675) |
| rig-A50 | Dark red | 6.23 ± 0.04 | pqqE (172) |
| rig-A54 | Dark red | 6.30 ± 0.07 | pqqE (172) |
| rig-A55 | Dark red | 6.32 ± 0.10 | pqqE (172) |
| rig-A112 | Dark red | 6.01 ± 0.38 | pqqE (172) |
| rig-A185 | Dark red | 6.11 ± 0.11 | pqqE (172) |
| rig-A228 | Dark red | ND | gdhS (1936) |
| rig-A237 | Dark red | 6.17 ± 0.04 | gdhS (1930) |
| rig-A243 | Dark red | 6.33 ± 0.14 | pqqE (686) |
| rig-A258 | Bright pink | 3.91 ± 0.22 | ubiB (736) |
a
All strains are in a Δcrp mutant background except rig-1 and rig-2, which are in a crp-1 background.
b
Color by visual inspection after growth in LBG medium for 20 h.
c
pH as determined from the culture medium after incubation at 20 h in LBG medium (n ≥ 3 experiments per reading). Values are means and standard deviations. ND, not determined.
d
NA, not applicable. Numbers in parentheses indicate the insertion sites of the transposon with respect to the first base pair of the open reading frame.