Fig 4.

Oxidation activities of E. coli cells expressing the wild-type CYP199A2 or its F185 mutants for coumaric and cinnamic acids. Whole cells were reacted with p-coumaric acid (A), m-coumaric acid (B), or cinnamic acid (C). (A and B) Initial rate of caffeic acid production, which was estimated for the first 30 min of the reaction; (C) initial rates of m-coumaric, p-coumaric, and caffeic acid production (white, black, and gray bars, respectively). Data are averages from three independent experiments, and error bars indicate standard deviations from the means. The molecular structures are shown as insets.