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(A and B) Spectral characterization of CDH IIA (A) and CDH IIB (B) showing the oxidized (black) and reduced (gray) spectra. Lactose was used for reduction. The difference spectra (oxidized-reduced) are given in the insets. (C) Spectrum of oxidized GH61-3, with the inset showing the spectrum of the type 2 copper atom in its oxidized (black) and reduced (gray) states. Ascorbate was used to reduce the enzyme.
