Table 2.
Population genetic analysis of 35 SSR loci in European and North American populations of Plasmopara viticola based on the genotyping of 96 isolatesa
Locus | Total Na | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Europe |
North America |
||||||||||||
Global |
HO for Gironde (France) | HO for Rhine Valley (Germany) | HO for Vaucluse (France) | Global |
HO for Michigan | HO for Virginia | HO for New York | ||||||
Ni | Na | HO | Ni | Na | HO | ||||||||
Pv61 | 4 | 41 | 3 | 0.073 | 0.062 | 0.125 | 43 | 4 | 0.349 | 0.071 | 0.333 | 0.643 | |
Pv65 | 5 | 42 | 2 | 0.523 | 0.562 | 0.437 | 0.562 | 25 | 4 | 0.24* | 0.333 | 0.429 | 0.133* |
Pv67 | 3 | 40 | 2 | 0.275 | 0.2 | 0.25 | 0.467 | 36 | 3 | 0.111 | 0.187 | 0.1 | |
Pv74 | 4 | 38 | 2 | 0.026* | 0* | 0.071 | 17 | 3 | 0.059* | 0.25 | |||
Pv76 | 4 | 37 | 1 | 37 | 3 | 0.027* | 0.071* | ||||||
Pv83 | 4 | 41 | 2 | 0.073 | 0.067 | 0.125 | 38 | 4 | 0.211* | 0.111 | 0.333 | 0.143 | |
Pv87 | 2 | 42 | 1 | 45 | 2 | 0* | |||||||
Pv88 | 4 | 40 | 2 | 0.15 | 0.286 | 0.062 | 0.062 | 44 | 3 | 0.25 | 0.267 | 0.467 | |
Pv91 | 3 | 42 | 2 | 0.261 | 0.125 | 0.562 | 45 | 3 | 0.178 | 0.25 | 0.267 | ||
Pv93 | 4 | 41 | 3 | 0.317 | 0.133 | 0.562 | 0.125 | 33 | 2 | 0.333 | 0.25 | 0.467 | |
Pv96 | 1 | 42 | 1 | 32 | 1 | ||||||||
Pv100 | 1 | 31 | 1 | 0 | 0 | ||||||||
Pv101 | 3 | 42 | 2 | 0.595 | 0.562 | 0.5 | 0.875 | 27 | 3 | 0.259* | 0.091 | 0.75 | |
Pv102 | 1 | 30 | 1 | 0 | 0 | ||||||||
Pv103 | 4 | 42 | 2 | 0.309 | 0.312 | 0.5 | 46 | 3 | 0.283* | 0.312 | 0.067 | 0.467 | |
Pv104 | 2 | 42 | 1 | 39 | 2 | 0.051* | 0.2 | ||||||
Pv124 | 2 | 42 | 1 | 47 | 2 | 0.17 | 0.437 | 0.067 | |||||
Pv126 | 6 | 42 | 2 | 0.024 | 0.062 | 42 | 6 | 0.547* | 0.429 | 0.6 | 0.615 | ||
Pv127 | 5 | 39 | 3 | 0.051 | 0.143 | 35 | 5 | 0.657 | 0.692 | 0.6 | 0.667 | ||
Pv133 | 2 | 41 | 1 | 42 | 2 | 0.167 | 0.467 | ||||||
Pv134 | 4 | 42 | 1 | 43 | 4 | 0.349* | 0.333 | 0.467 | 0.231 | ||||
Pv135 | 2 | 42 | 2 | 0.048 | 0.125 | 45 | 2 | 0.467 | 0.867 | 0.2 | 0.333 | ||
Pv136 | 2 | 41 | 1 | 38 | 2 | 0* | |||||||
Pv137 | 6 | 41 | 3 | 0.585 | 0.625 | 0.533 | 0.562 | 28 | 5 | 0.393* | 0.143 | 0.571 | 0.286 |
Pv138 | 6 | 40 | 5 | 0.25* | 0.312 | 0.312* | 38 | 6 | 0.263* | 0.154 | 0.467 | 0.1 | |
Pv139 | 4 | 41 | 3 | 0.146 | 0.375 | 45 | 3 | 0.156 | 0.133 | 0.357 | |||
Pv140 | 9 | 41 | 8 | 0.683 | 0.867 | 0.437 | 0.75 | 44 | 7 | 0.454* | 0.333 | 0.467 | 0.571 |
Pv141 | 2 | 42 | 2 | 0.548 | 0.562 | 0.375 | 0.875 | 45 | 2 | 0.511 | 0.733 | 0.8 | |
Pv142 | 5 | 42 | 2 | 0.238 | 0.375 | 0.125 | 0.312 | 13 | 5 | 0.308* | 1 | 0.333 | |
Pv143 | 5 | 42 | 3 | 0.548 | 0.687 | 0.625 | 0.312 | 44 | 4 | 0.25* | 0.214 | 0.067 | 0.467 |
Pv144 | 16 | 42 | 11 | 0.667* | 0.75 | 0.812 | 0.375* | 44 | 11 | 0.204* | 0.4 | 0.214* | 0* |
Pv145 | 1 | 42 | 1 | 45 | 1 | ||||||||
Pv146 | 2 | 42 | 1 | 34 | 2 | 0.206 | 0.111 | 0.429 | |||||
Pv147 | 6 | 42 | 5 | 0.476 | 0.562 | 0.562 | 0.125 | 46 | 2 | 0.196* | 0.067 | 0.187 | 0.333 |
Pv148 | 2 | 42 | 2 | 0.286 | 0.187 | 0.25 | 0.5 | 43 | 2 | 0.372 | 0.357 | 0.429 | 0.333 |
For each population, 16 isolates were genotyped. Ni, number of isolates amplified; Na, number of alleles; HO, observed heterozygosity; *, significant deviation from the Hardy-Weinberg equilibrium test after Bonferroni's correction for multiple tests (P < 0.0014).