Fig 5.
Detection of internalized human NoV GII.4 RNA in romaine lettuce grown hydroponically. Feed water was inoculated with human NoV GII.4 stock to a starting titer on day 0 of 2.9 × 106 RNA copies/ml. The titer of the feed water was monitored throughout the 14-day study period and is reported as RNA copies/ml. At each time point, roots, shoots, and leaves of romaine lettuce were harvested, homogenized with liquid nitrogen and mortar and pestle, and resuspended in 5 ml of sterile PBS. Sample homogenate was then subjected to centrifugation at 1,000 × g for 30 min, and the supernatant was tested for the presence of human NoV RNA. Data shown in the internalization kinetics plot were determined by RT-qPCR, and results are reported as RNA copies/g. Data points are the averages of three replicates. Error bars represent ±1 standard deviation.