Fig 8.

Detection of internalized human NoV RNA in romaine lettuce treated with RNase. Amounts of 100 μl of processed lettuce samples from the experiment whose results are shown in Fig. 5 were treated with 5 μg of RNase at 37°C for 1 h. The RNA was extracted using an RNeasy kit (Qiagen), followed by real-time RT-PCR. Results are reported as RNA copies/g. Data points are the averages of three replicates. Error bars represent ±1 standard deviation. Statistical differences (P < 0.05) are indicated by an asterisk.