Table 1.
Genotypes of MAP isolates in this study and their origins
| |
|
Genotyping method |
|
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Isolate No. | Genotype No. |
No. of MIRU repeats (Product size) |
Short sequence repeats (Number) |
IS1311/REA type | Herd code | DISTRICT | ||||
| MIRU 2 | MIRU 3 | VNTR 32 | Locus 1 | Locus 2 | Locus 8 | |||||
| MapUg1 |
1 |
9(350) |
1(198) |
- |
- |
12 G |
GGTtgtGGT |
B |
020 |
Wakiso |
| MapUg2 |
2 |
15(520) |
1(198) |
- |
- |
11 G |
GGTtgtGGT |
X |
058 |
Luwero |
| MapUg3 |
3 |
- |
- |
- |
- |
11 G |
GGTtgtGGT |
B |
013 |
Wakiso |
| MapUg4 |
4 |
9(350) |
5(300) |
9(300) |
- |
11 G |
GGTtgtGGT |
X |
018 |
Wakiso |
| MapUg5 |
5 |
9(350) |
5(300) |
9(300) |
- |
10 G |
GGTcgtcgt |
C |
022 |
Wakiso |
| MapUg6_ |
6 |
15(520) |
5(300) |
9(300) |
6 G |
10 G |
GGTtgtGGT |
C |
023 |
Wakiso |
| MapUg7 |
7 |
7(300) |
5(300) |
9(300) |
7 G |
10 G |
4GGT |
C |
030 |
Wakiso |
| MapUg8 |
8 |
15(520) |
5(300) |
9(300) |
7 G |
11 G |
GGTtgtGGT |
C |
044 |
Luwero |
| MapUg9 |
8 |
15(520) |
5(300) |
9(300) |
7 G |
11 G |
GGTtgtGGT |
C |
038 |
Mpigi |
| MapUg10* |
9 |
15(520) |
5(300) |
9(300) |
- |
11 G |
GGTtgtGGT |
C |
033 |
Mpigi |
| MapUg11 |
10 |
9(350) |
5(300) |
9(300) |
6 G |
10 G |
4GGT |
C |
048 |
Masindi |
| MapUg12 |
10 |
9(350) |
5(300) |
9(300) |
6 G |
10 G |
4GGT |
C |
Abattoir |
Kampala |
| MapUg13 |
10 |
9(350) |
5(300) |
9(300) |
6 G |
10 G |
4GGT |
C |
Abattoir |
Kampala |
| MapUg14 |
10 |
9(350) |
5(300) |
9(300) |
6 G |
10 G |
4GGT |
C |
Abattoir |
Kampala |
| MapUg15 |
10 |
9(350) |
5(300) |
9(300) |
6 G |
10 G |
4GGT |
C |
Abattoir |
Kampala |
| MapUg16 |
10 |
9(350) |
5(300) |
9(300) |
6 G |
10 G |
4GGT |
C |
Abattoir |
Kampala |
| MapUg17 |
10 |
9(350) |
5(300) |
9(300) |
6 G |
10 G |
4GGT |
C |
Abattoir |
Kampala |
| MapUg18 |
10 |
9(350) |
5(300) |
9(300) |
6 G |
10 G |
4GGT |
C |
Abattoir |
Kampala |
| MapUg19 |
10 |
9(350) |
5(300) |
9(300) |
6 G |
10 G |
4GGT |
C |
Abattoir |
Kampala |
| MapUg20 |
10 |
9(350) |
5(300) |
9(300) |
6 G |
10 G |
4GGT |
C |
Abattoir |
Kampala |
| MapUg21 | 11 | 9(350) | 5(300) | 9(300) | 6 G | 10 G | 2GGT | C | Abattoir | Kampala |
Genotype profiles of the 21 isolates. Note that isolates whose MIRUs, VNTR 32, SSR loci 1, 2 could not be amplified had distinct genotype profiles. These isolates were confirmed to belong to different genotypes using the SSR locus 8 phyologenetic analysis.
* MapUg10 belonged to a different genotype according to SSR locus 8 SNP phylogenetic analysis.