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Construction of the basic structural elements. (A) Antisense RNAs targeted to HIV-1 env were used as linkers, and the positions in the env gene are indicated. (B) Inhibition of the structure elements. Firefly luciferase activity in the cell was measured 2 days post-transfection and normalized to the Renilla luciferase activity. Firefly luciferase activity in the presence of miR-LacZ was normalized at 100%.
